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antibody anti rat cd2  (Bio-Rad)


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    Bio-Rad antibody anti rat cd2
    Antibody Anti Rat Cd2, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 197 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/antibody+anti+rat+cd2/Mouse+anti+Rat+CD2/10__7554_slash_elife__104723__3-136-64-70
    Average 93 stars, based on 197 article reviews
    antibody anti rat cd2 - by Bioz Stars, 2026-09
    93/100 stars

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    Article Title: Numb provides a fail-safe mechanism for intestinal stem cell self-renewal in adult Drosophila midgut
    Article Snippet: DOI: https://doi.org/10.7554/eLife.104723 13 of 17 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Genetic reagent (D. melanogaster) FRT 19A, ubi- mRFPnls Bloomington Drosophila Stock Center BDSC 31418 FLYB: FBst0031418 RRID:BDSC_31418 FlyBase symbol: P{w[+mC]= Ubi- mRFP. nls}1, w[*], P{ry[+t7.2]=hsFLP}12P{ry[+t7.2]=neoFRT}19A Antibody anti- GFP (chicken polyclonal) Abcam Cat#: ab13970; RRID:AB_300798 IF (1:1000) Antibody anti-β-Galactosidase (rabbit polyclonal) MP Biomedicals Cat#: 08559761 RRID:AB_3675281 IF (1:1000) Antibody anti- Rat CD2 (mouse monoclonal) Bio- Rad Cat#: MCA154GA RRID:AB_566608 IF (1:2000) (Formerly AbD Serotec) Antibody anti- Dl extracellular domain (mouse monoclonal) DSHB Cat#: c594.9b RRID:AB_528194 IF (1:20) Antibody anti- Prospero (mouse monoclonal) DSHB Cat#: Prospero RRID:AB_528440 IF (1:20) Antibody anti- Pdm1 (rabbit polyclonal) Dr. Xiaohang Yang IF (1:1000) Antibody anti- Chicken Alexa Fluor 488 (goat polyclonal secondary) Thermo Fisher Scientific Cat#: A- 11039 RRID:AB_2534096 IF (1:1000) Antibody anti- Mouse Alexa Flour 546 (goat polyclonal secondary) Thermo Fisher Scientific Cat#: A- 11030 RRID:AB_2737024 IF (1:1000) Antibody anti- Rabbit Alexa Flour 546 (goat polyclonal secondary) Thermo Fisher Scientific Cat#: A- 11035 RRID:AB_2534093 IF (1:1000) Antibody anti- Mouse Cy5 (goat polyclonal) Jackson ImmunoResearch Labs Cat#: 115- 175- 166 RRID:AB_2338714 IF (1:500) Antibody anti- Rabbit Cy5 (goat polyclonal) Jackson ImmunoResearch Labs Cat#: 111- 175- 144 RRID:AB_2338013 IF (1:500) Chemical compound, drug DAPI (4′,6- diamidino2- phenylindole, dihydrochloride) Invitrogen Cat#: D1306 IF (1:2000) Chemical compound, drug DSS (dextran sulfate sodium) Sigma- Aldrich Cat#: 42867 5% solution Chemical compound, drug Bleomycin sulfate from Streptomyces verticillus Sigma- Aldrich Cat#: B8416 25 μg/ml Chemical compound, drug Sucrose Fisher Bioreagent Cat#: BP220- 212 5% solution Continued Drosophila genetics and transgenes Flies were maintained on cornmeal at 25°C.



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    Pc positively regulates Sens expression by regulating H4K20me1 catalyzed by PR-Set7. (A - A''') A wing disc carrying PR-Set7 EY04668 mutant clones and immunostained with anti-Sens antibody (red), <t>anti-CD2</t> (green) and anti-H4K20me1 antibody (blue). The clones are CD2 negative, and their boundary is demarcated with dashed lines. (B - C) RT-qPCR analysis of mRNA levels of PR-Set7 (B) and Sens (C) in Act5c-Gal4 or Act5c-Gal4/uas-dsRNA PR-Set7 wing discs. (D) ChIP-qPCR analysis using control IgG or anti-H4K20me1 antibody at the Sens locus with Act5c-Gal4 wing discs or Act5c-Gal4/uas-dsRNA PR-Set7 wing discs. Upper panel illustrates the positions of the primers used for the qPCR assay at the Sens locus. In the lower panel, ChIP signal levels are represented as percentages of input chromatin. (E) ChIP-qPCR analysis using control IgG or anti-H4K20me1 antibody with Act5c-Gal4 wing discs or Act5c-Gal4/uas-dsRNA Pc wing discs at the Sens locus. ChIP signal levels are represented as percentages of input chromatin. (F) S2 cells were transfected with combinations of DNA constructs as indicated. After 48 h of transfection, lysates from transfected S2 cells were immunoprecipitated with anti-Flag antibody or anti-Myc antibody. Western blots were performed to analyze the presence of Flag- or Myc-tagged proteins. (G - H) Mapping of individual domains responsible for the interaction of PR-Set7 with Pc. Schematic drawings of PR-Set7 full length (FL) protein and different fragments (G) . The SET domain that catalyzes H4K20me1 is located in the C-terminal (C) part of PR-Set7, while no significant domain is in the N-terminal (N) and middle (M) regions. A co-IP-WB shows that PR-Set7 interacts with Pc mainly through its M region (H) . Means ± SD; n = 3. n.s., no significant difference. * P < 0.05, ** P < 0.01, *** P < 0.001. Unpaired, two-tailed Student's t -test. See also .
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    Pc positively regulates Sens expression by regulating H4K20me1 catalyzed by PR-Set7. (A - A''') A wing disc carrying PR-Set7 EY04668 mutant clones and immunostained with anti-Sens antibody (red), <t>anti-CD2</t> (green) and anti-H4K20me1 antibody (blue). The clones are CD2 negative, and their boundary is demarcated with dashed lines. (B - C) RT-qPCR analysis of mRNA levels of PR-Set7 (B) and Sens (C) in Act5c-Gal4 or Act5c-Gal4/uas-dsRNA PR-Set7 wing discs. (D) ChIP-qPCR analysis using control IgG or anti-H4K20me1 antibody at the Sens locus with Act5c-Gal4 wing discs or Act5c-Gal4/uas-dsRNA PR-Set7 wing discs. Upper panel illustrates the positions of the primers used for the qPCR assay at the Sens locus. In the lower panel, ChIP signal levels are represented as percentages of input chromatin. (E) ChIP-qPCR analysis using control IgG or anti-H4K20me1 antibody with Act5c-Gal4 wing discs or Act5c-Gal4/uas-dsRNA Pc wing discs at the Sens locus. ChIP signal levels are represented as percentages of input chromatin. (F) S2 cells were transfected with combinations of DNA constructs as indicated. After 48 h of transfection, lysates from transfected S2 cells were immunoprecipitated with anti-Flag antibody or anti-Myc antibody. Western blots were performed to analyze the presence of Flag- or Myc-tagged proteins. (G - H) Mapping of individual domains responsible for the interaction of PR-Set7 with Pc. Schematic drawings of PR-Set7 full length (FL) protein and different fragments (G) . The SET domain that catalyzes H4K20me1 is located in the C-terminal (C) part of PR-Set7, while no significant domain is in the N-terminal (N) and middle (M) regions. A co-IP-WB shows that PR-Set7 interacts with Pc mainly through its M region (H) . Means ± SD; n = 3. n.s., no significant difference. * P < 0.05, ** P < 0.01, *** P < 0.001. Unpaired, two-tailed Student's t -test. See also .
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    Pc positively regulates Sens expression by regulating H4K20me1 catalyzed by PR-Set7. (A - A''') A wing disc carrying PR-Set7 EY04668 mutant clones and immunostained with anti-Sens antibody (red), anti-CD2 (green) and anti-H4K20me1 antibody (blue). The clones are CD2 negative, and their boundary is demarcated with dashed lines. (B - C) RT-qPCR analysis of mRNA levels of PR-Set7 (B) and Sens (C) in Act5c-Gal4 or Act5c-Gal4/uas-dsRNA PR-Set7 wing discs. (D) ChIP-qPCR analysis using control IgG or anti-H4K20me1 antibody at the Sens locus with Act5c-Gal4 wing discs or Act5c-Gal4/uas-dsRNA PR-Set7 wing discs. Upper panel illustrates the positions of the primers used for the qPCR assay at the Sens locus. In the lower panel, ChIP signal levels are represented as percentages of input chromatin. (E) ChIP-qPCR analysis using control IgG or anti-H4K20me1 antibody with Act5c-Gal4 wing discs or Act5c-Gal4/uas-dsRNA Pc wing discs at the Sens locus. ChIP signal levels are represented as percentages of input chromatin. (F) S2 cells were transfected with combinations of DNA constructs as indicated. After 48 h of transfection, lysates from transfected S2 cells were immunoprecipitated with anti-Flag antibody or anti-Myc antibody. Western blots were performed to analyze the presence of Flag- or Myc-tagged proteins. (G - H) Mapping of individual domains responsible for the interaction of PR-Set7 with Pc. Schematic drawings of PR-Set7 full length (FL) protein and different fragments (G) . The SET domain that catalyzes H4K20me1 is located in the C-terminal (C) part of PR-Set7, while no significant domain is in the N-terminal (N) and middle (M) regions. A co-IP-WB shows that PR-Set7 interacts with Pc mainly through its M region (H) . Means ± SD; n = 3. n.s., no significant difference. * P < 0.05, ** P < 0.01, *** P < 0.001. Unpaired, two-tailed Student's t -test. See also .

    Journal: Cell Research

    Article Title: A positive role for polycomb in transcriptional regulation via H4K20me1

    doi: 10.1038/cr.2016.33

    Figure Lengend Snippet: Pc positively regulates Sens expression by regulating H4K20me1 catalyzed by PR-Set7. (A - A''') A wing disc carrying PR-Set7 EY04668 mutant clones and immunostained with anti-Sens antibody (red), anti-CD2 (green) and anti-H4K20me1 antibody (blue). The clones are CD2 negative, and their boundary is demarcated with dashed lines. (B - C) RT-qPCR analysis of mRNA levels of PR-Set7 (B) and Sens (C) in Act5c-Gal4 or Act5c-Gal4/uas-dsRNA PR-Set7 wing discs. (D) ChIP-qPCR analysis using control IgG or anti-H4K20me1 antibody at the Sens locus with Act5c-Gal4 wing discs or Act5c-Gal4/uas-dsRNA PR-Set7 wing discs. Upper panel illustrates the positions of the primers used for the qPCR assay at the Sens locus. In the lower panel, ChIP signal levels are represented as percentages of input chromatin. (E) ChIP-qPCR analysis using control IgG or anti-H4K20me1 antibody with Act5c-Gal4 wing discs or Act5c-Gal4/uas-dsRNA Pc wing discs at the Sens locus. ChIP signal levels are represented as percentages of input chromatin. (F) S2 cells were transfected with combinations of DNA constructs as indicated. After 48 h of transfection, lysates from transfected S2 cells were immunoprecipitated with anti-Flag antibody or anti-Myc antibody. Western blots were performed to analyze the presence of Flag- or Myc-tagged proteins. (G - H) Mapping of individual domains responsible for the interaction of PR-Set7 with Pc. Schematic drawings of PR-Set7 full length (FL) protein and different fragments (G) . The SET domain that catalyzes H4K20me1 is located in the C-terminal (C) part of PR-Set7, while no significant domain is in the N-terminal (N) and middle (M) regions. A co-IP-WB shows that PR-Set7 interacts with Pc mainly through its M region (H) . Means ± SD; n = 3. n.s., no significant difference. * P < 0.05, ** P < 0.01, *** P < 0.001. Unpaired, two-tailed Student's t -test. See also .

    Article Snippet: Antibodies used in this study were rat anti-Ci antibody (DSHB), rabbit anti-Pc antibody (which was generated in this study by using the peptide RKAEVLKESGKIG), mouse anti-Ubx antibody (DSHB), guinea pig anti-Sens antibody (a kind gift from Dr Hugo J Bellen), rabbit anti-H3K27me3 antibody (Millipore), mouse anti-CD2 antibody (AbD Serotec), rabbit anti-H4K20me1 antibody (Abcam), and secondary antibodies were bought from Millipore.

    Techniques: Expressing, Mutagenesis, Clone Assay, Quantitative RT-PCR, ChIP-qPCR, Control, Transfection, Construct, Immunoprecipitation, Western Blot, Co-Immunoprecipitation Assay, Two Tailed Test